Anti-SNAIL + SLUG antibody
| Name | Anti-SNAIL + SLUG antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab85936 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Human, Rat |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SNAIL |
| Description | Rabbit Polyclonal |
| Gene | SNAI2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-SNAIL + SLUG antibody (ab85936) at 1 µg/mlLane 1 : SLUG (SNAI2) Human - GST Tagged Recombinant Protein Lane 2 : SNAIL (SNAI1) - GST Tagged Recombinant Protein Lysates/proteins at 0.1 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of ab85936 staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85936, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Mutant PIK3CA accelerates HER2-driven transgenic mammary tumors and induces - Mutant PIK3CA accelerates HER2-driven transgenic mammary tumors and induces
Hanker AB, Pfefferle AD, Balko JM, Kuba MG, Young CD, Sanchez V, Sutton CR, Cheng H, Perou CM, Zhao JJ, Cook RS, Arteaga CL. Proc Natl Acad Sci U S A. 2013 Aug 27;110(35):14372-7. doi:
Epithelial to mesenchymal transition is activated in metastatic pheochromocytomas - Epithelial to mesenchymal transition is activated in metastatic pheochromocytomas
Loriot C, Burnichon N, Gadessaud N, Vescovo L, Amar L, Libe R, Bertherat J, Plouin PF, Jeunemaitre X, Gimenez-Roqueplo AP, Favier J. J Clin Endocrinol Metab. 2012 Jun;97(6):E954-62.
Inhibition of CCN6 (Wnt-1-induced signaling protein 3) down-regulates E-cadherin - Inhibition of CCN6 (Wnt-1-induced signaling protein 3) down-regulates E-cadherin
Huang W, Zhang Y, Varambally S, Chinnaiyan AM, Banerjee M, Merajver SD, Kleer CG. Am J Pathol. 2008 Apr;172(4):893-904.