Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 200X.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 400X.
ab180714 staining SNAIL in human MCF10A cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 3.7% PFA in PBS for 15 minutes and blocked with 0.1% Milk + 0.1% Tween in PBS for 30 minutes at 25°C. Samples were incubated with primary antibody (1/1000 in PBS + 0.1% Tween) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview
ab180714 staining SNAIL in mouse embryonic heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100 in blocking buffer) for 16 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
Anti-SNAIL antibody (ab180714) at 1/500 dilution + Mouse heart tissue extracts