SNAI1

Gene Symbol SNAI1
Entrez Gene 6615
Alt Symbol SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1
Species Human
Gene Type protein-coding
Description snail family zinc finger 1
Other Description protein sna|protein snail homolog 1|snail 1 homolog|snail 1 zinc finger protein|snail 1, zinc finger protein|snail homolog 1|zinc finger protein SNAI1
Swissprots Q9P113 Q9UHH7 B2R842 O95863 Q9UBP7
Accessions AAD52986 AAD52996 CAB52414 CBX55848 EAW75640 O95863 AF125377 AAD17332 AF131208 AAF32527 AK313228 BAG36039 BC012910 AAH12910 BI822180 DA972913 DQ891599 ABM82525 DQ894793 ABM85719 NM_005985 NP_005976
Function Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up- regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself. {ECO:0000269|PubMed:10655587, ECO:0000269|PubMed:15647282, ECO:0000269|PubMed:16096638, ECO:0000269|PubMed:20121949, ECO:0000269|PubMed:20562920, ECO:0000269|PubMed:21952048}.
Subcellular Location Nucleus. Cytoplasm. Note=Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
Tissue Specificity Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. {ECO:0000269|PubMed:10655587}.
Top Pathways Adherens junction

Snail (L70G2) Mouse mAb - 3895 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone L70G2
Western blot analysis of extracts from HCT-116, NIH/3T3 and Rat2 cells using Snail (L70G2) Mouse mAb.

Snail (SN9H2) Rat mAb - 4719 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rat
Clonality Monoclonal
Clone SN9H2
Western blot analysis of total cell lysates from HCT116, 293 cells, and 293 cells transiently transfected with a SNAIL expressing construct, using Snail (SN 9H2) Rat mAb.

Snail (C15D3) Rabbit mAb - 3879 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C15D3
Western blot analysis of extracts from various cell lines using Snail (C15D3) Rabbit mAb.

SNAI 1 (E-10) - sc-393172 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SNAI 1 (E-10): sc-393172. Western blot analysis of SNAI 1 expression in non-transfected: sc-117752 (A) and human SNAI 1 transfected: sc-113766 (B) 293T whole cell lysates.

SNAI 1 (G-7) - sc-271977 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SNAI 1 (G-7): sc-271977. Western blot analysis of SNAI 1 expression in non-transfected: sc-117752 (A) and human SNAI 1 transfected: sc-113766 (B) 293T whole cell lysates.

SNAI 1 (H-130) - sc-28199 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SNAI 1 (H-130): sc-28199. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

SNAI 1 (E-18) - sc-10432 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SNAI 1 (E-18): sc-10432. Western blot analysis of SNAI 1 expression in non-transfected: sc-117752 (A) and human SNAI 1 transfected: sc-113766 (B) 293T whole cell lysates.

SNAI 1 (T-18) - sc-10433 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SNAI 1 (T-18): sc-10433. Western blot analysis of SNAI 1 expression in non-transfected: sc-117752 (A) and human SNAI 1 transfected: sc-113766 (B) 293T whole cell lysates.

Anti-SNAIL (phospho S246) antibody - ab182654 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SNAIL (phospho S246) antibody (ab182654) at 1/500 dilutionLane 1 : Rat heart tissue extractLane 2 : Rat heart tissue extract with antigen-specific peptide

Anti-SNAIL antibody - ab180714 from Abcam

Prices $392.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 200X.

Anti-SNAIL antibody - ab53519 from Abcam

Prices $382.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
All lanes : Anti-SNAIL antibody (ab53519) at 1/500 dilutionLane 1 : SNAIL (SNAI1) Human Recombinant Protein Lane 2 : SLUG (SNAI2) Human Recombinant ProteinLysates/proteins at 0.1 µg per lane.SecondaryRabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 68 kDa (why is the actual band size different from the predicted?)Exposure time : 12 minutes

Anti-SNAIL antibody - ab82846 from Abcam

Prices $382.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SNAIL antibody (ab82846) at 1/200 dilutionLane 1 : SNAIL (SNAI1) Human Recombinant Protein Lane 2 : SLUG (SNAI2) Human Recombinant ProteinLysates/proteins at 0.1 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-SNAIL antibody - ab167609 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-SNAIL antibody (ab167609) at 1 µg/mlLane 1 : SNAIL transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat anti-Mouse IgG

Anti-SNAIL antibody - ab110490 from Abcam

Prices $376.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SNAIL antibody (ab110490) at 1/250 dilutionLane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate - TGF-beta TreatedLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-SNAIL antibody - ab167615 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-SNAIL antibody (ab167615) at 1 µg/mlLane 1 : SNAIL transfected 293T cell lysate (29.15 KDa).Lane 2 : Non-transfected 293T cell lysate.Lysates/proteins at 15 µl per lane.

Anti-SNAIL antibody - ab196885 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-SNAIL antibody (ab196885) at 1/500 dilutionLane 1 : Ht29 cell extractLane 2 : Ht29 cell extract with synthesized peptide

Anti-SNAIL antibody [10D7] - ab117866 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 10D7
All lanes : Anti-SNAIL antibody [10D7] (ab117866) at 1/1000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY SNAIL cDNA Lysates/proteins at 5 µg per lane.

Anti-SNAIL antibody [6D2] - ab130245 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6D2
Anti-SNAIL antibody [6D2] (ab130245) at 1/500 dilution + Recombinant Human SNAIL protein

Anti-SNAIL antibody [Sn9H2] - ab31787 from Abcam

Prices $378.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone Sn9H2
All lanes : Anti-SNAIL antibody [Sn9H2] (ab31787) at 10 µg/mlLane 1 : SNAIL (SNAI1) Human Recombinant Protein Lane 2 : SLUG (SNAI2) Human Recombinant Protein Lysates/proteins at 0.1 µg per lane.SecondaryPeroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-SNAIL antibody [Sn9H2] - BSA and Azide free - ab175375 from Abcam

Prices $370.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone Sn9H2

Anti-SNAIL antibody - N-terminal - ab135507 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-SNAIL antibody - N-terminal (ab135507) at 1/50 dilution + NCI-H460 cell line lysate at 35 µg

Anti-SNAI1 / SNAIL-1 Antibody - LS-C39148 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rat
Clonality Monoclonal

Anti-SNAI1 / SNAIL-1 Antibody (aa8-19) - LS-C55492 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-SNAI1 / SNAIL-1 Antibody (N-Terminus) - LS-C29804 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SNAI1 / SNAIL antibody LS-C29804 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.

Anti-SNAI1 / SNAIL-1 Antibody (clone 5E12) - LS-C114440 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5E12
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAI1.
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