
All lanes : Anti-SNAIL antibody (ab110490) at 1/250 dilutionLane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate - TGF-beta TreatedLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

All lanes : Anti-SNAIL antibody (ab110490) at 1/250 dilutionLane 1 : MCF7 lysate overexpressing SNAIL proteinLane 2 : MCF7 cell lysate overexpressing SLUG proteinLane 3 : MCF7 lysate transfected with vector control plasmidLane 4 : Untransfected MCF7 LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.