Anti-SNAIL antibody [10D7]
| Name | Anti-SNAIL antibody [10D7] |
|---|---|
| Supplier | Abcam |
| Catalog | ab117866 |
| Prices | $376.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 10D7 |
| Applications | FC WB |
| Species Reactivities | Human |
| Antigen | Protein expressed in 293T cells, transfected with Human SNAIL (NM_005985) expression vector |
| Description | Mouse Monoclonal |
| Gene | SNAI1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-SNAIL antibody [10D7] (ab117866) at 1/1000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY SNAIL cDNA Lysates/proteins at 5 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/2554_SNAIL-Primary-antibodies-ab117866-1.jpg)
All lanes : Anti-SNAIL antibody [10D7] (ab117866) at 1/1000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY SNAIL cDNA Lysates/proteins at 5 µg per lane.
![Overlay histogram showing HCT116 cells stained with ab117866 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab117866, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/2555_SNAIL-Primary-antibodies-ab117866-2.jpg)
Overlay histogram showing HCT116 cells stained with ab117866 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab117866, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.