Anti-SNAP25 antibody
| Name | Anti-SNAP25 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5666 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP ICC/IF ICC/IF WB ICC/IF IHC-F |
| Species Reactivities | Mouse, Rat, Chicken, Guinea Pig, Bovine, Zebrafish, Human, Chimpanzee, Monkey, Fish |
| Antigen | Synthetic peptide corresponding to Mouse SNAP25 aa 195-206 |
| Description | Rabbit Polyclonal |
| Gene | SNAP25 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunlocalization of SNAP-25 in cultured rat hippocampal cells using ab5666.
Anti-SNAP25 antibody (ab5666) at 1/10000 dilution + Whole tissue lysate prepared from mouse hippocampus.SecondaryHRP conjugated goat anti-rabbit Ig. at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 25 kDa (why is the actual band size different from the predicted?)Exposure time : 20 secondsThis image is courtesy of an anonymous AbreviewSee Abreview
ICC/IF image of ab5666 stained PC12 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5666, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
SNAP25 was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to SNAP25 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab5666.Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.Band: 25kDa; SNAP25
Product References
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