SNAP25

Gene Symbol SNAP25
Entrez Gene 6616
Alt Symbol RIC-4, RIC4, SEC9, SNAP, SNAP-25, bA416N4.2, dJ1068F16.2
Species Human
Gene Type protein-coding
Description synaptosomal-associated protein, 25kDa
Other Description SUP|resistance to inhibitors of cholinesterase 4 homolog|super protein|synaptosomal-associated protein 25|synaptosomal-associated protein, 25kD
Swissprots D3DW17 B2RAU4 Q53EM2 Q5U0B5 P60880 D3DW16
Accessions EAX10346 EAX10347 EAX10348 EAX10349 EAX10350 EAX10351 EAX10352 EAX10353 P60880 AK026732 AK090857 AK094560 AK098347 AK098767 AK223617 BAD97337 AK289647 BAF82336 AK314359 BAG36991 AM393230 CAL38108 AM393653 CAL38529 BC010647 AAH10647 BI552777 BI602062 BI604120 BT019684 AAV38490 BX647443 D21267 BAA22370 HQ447952 ADQ32436 L19760 AAC37545 L19761 AAC37546 XM_005260808 XP_005260865 XM_005260810 XP_005260867 NM_003081 NP_003072 NM_130811 NP_570824
Function t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
Subcellular Location Cytoplasm, perinuclear region {ECO:0000250}. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Note=Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region (By similarity). {ECO:0000250}.
Tissue Specificity Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Top Pathways Synaptic vesicle cycle

SNAP25 (D7B4) Rabbit mAb - 5308 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7B4
Western blot analysis of extracts from mouse and rat brain using SNAP25 (D7B4) Rabbit mAb.

SNAP25 (D9A12) Rabbit mAb - 5309 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9A12
Western blot analysis of extracts from mouse and rat brain using SNAP25 (D9A12) Rabbit mAb.

SNAP25 (A195) Antibody - 4117 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from mouse and rat brain using SNAP25 (A195) Antibody.

SNAP25 (D110) Antibody - 3926 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from mouse and rat brain using SNAP25 (D110) Antibody.

SNAP 25 (H-1) - sc-376713 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SNAP 25 (H-1): sc-376713. Western blot analysis of SNAP 25 expression in SH-SY5Y whole cell lysate.

SNAP 25 (C-18) - sc-7538 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SNAP 25 (C-18): sc-7538. Western blot analysis of SNAP 25 expression in PC-12 whole cell lysate (A) and in rat cerebellum (B) and whole rat brain (C) extracts. Note presence of undissociated SNAP 25-NF68kD complex at top of gel.

SNAP 25 (D-11) - sc-390874 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SNAP25 (D-11): sc-390874. Western blot analysis of SNAP 25 expression in SH-SY5Y (A) and PC-12 (B) whole cell lysates and mouse brain (C), rat brain (D) and human hippocampus (E) tissue extracts.

SNAP 25 (H-50) - sc-30094 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

SNAP 25 (N-19) - sc-7539 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SNAP 25 (N-19): sc-7539. Western blot analysis of SNAP 25 expression in non-transfected: sc-110760 (A) and human SNAP 25 transfected: sc-111113 (B) 293 whole cell lysates.

SNAP 25 (SP12) - sc-20038 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SNAP 25 (SP12): sc-20038. Western blot analysis of SNAP 25 expression in non-transfected: sc-110760 (A) and human SNAP 25 transfected: sc-111113 (B) 293 whole cell lysates.

SNAP 25 (SP14) - sc-65508 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SNAP 25 (SP14): sc-65508. Western blot analysis of SNAP 25 expression in non-transfected: sc-110760 (A) and human SNAP 25 transfected: sc-111113 (B) 293 whole cell lysates.

SNAP 25 (4E203) - sc-73044 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SNAP 25 (4E203): sc-73044. Western blot analysis of SNAP 25 expression in SH-SY5Y whole cell lysate.

SNAP 25 (20) - sc-136267 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
SNAP 25 (20): sc-136267. Western blot analysis of SNAP 25 expression in rat cerebrum tissue extract.

SNAP 25 (B-8) - sc-390644 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SNAP 25 (B-8): sc-390644. Western blot analysis of SNAP 25 expression in PC-12 whole cell lysate.

Anti-SNAP25 (phospho S187) antibody - ab169871 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SNAP25 (phospho S187) antibody (ab169871) at 1/1000 dilutionLane 1 : Rat hippocampal lysateLane 2 : Rat hippocampal lysate treated with Lambda phosphatase (400 units/100 µl lysate for 30 min)

Anti-SNAP25 antibody - ab5666 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunlocalization of SNAP-25 in cultured rat hippocampal cells using ab5666.

Anti-SNAP25 antibody - ab41455 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SNAP25 antibody (ab41455) at 1 µg/mlLane 1 : Spinal cord (Rat) tissue lysateLane 2 : Hippocampus (Mouse) tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-SNAP25 antibody - ab53723 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SNAP25 antibody (ab53723) at 1/500 dilutionLane 1 : RAW264.7 cell extracts, treated with EGF (200ng/ml, 30mins)Lane 2 : RAW264.7 cell extracts, treated with EGF (200ng/ml, 30mins) with immunizing peptide

Anti-SNAP25 antibody - ab66066 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
All lanes : Anti-SNAP25 antibody (ab66066) at 1 µg/mlLane 1 : SNAP25 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 50 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-SNAP25 antibody - ab31281 from Abcam

Prices $385.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ICC/IF image of ab31281 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31281 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SNAP25 antibody - ab116232 from Abcam

Prices $379.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-SNAP25 antibody (ab116232) at 0.03 µg/ml + Mouse brain lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-SNAP25 antibody - ab115655 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SNAP25 antibody - ab51008 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SNAP25 antibody - ab72394 from Abcam

Host Sheep
Clonality Polyclonal

Anti-SNAP25 antibody [4C6] - ab119028 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4C6
All lanes : Anti-SNAP25 antibody [4C6] (ab119028) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SNAP25 cDNALysates/proteins at 5 µg per lane.
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