Anti-SNAP25 antibody
| Name | Anti-SNAP25 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab41455 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP IHC-F WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Zebrafish, Chicken, Bovine |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human SNAP25 |
| Description | Rabbit Polyclonal |
| Gene | SNAP25 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-SNAP25 antibody (ab41455) at 1 µg/mlLane 1 : Spinal cord (Rat) tissue lysateLane 2 : Hippocampus (Mouse) tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
Anti-SNAP25 antibody (ab41455) at 1 µg/ml + Human SNAP25 full length protein (ab74529) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 1 minute
ICC/IF image of ab41455 stained human SH-SY5Y cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41455, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab41455 staining SNAP25 in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/3000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary. The picture shows the staining obtained at the level of the hippocampus. The staining was very strong, located in the whole brain.See Abreview
All lanes : Anti-SNAP25 antibody (ab41455) at 1 µg/mlLane 1 : MarkerLane 2 : Zebrafish brain homogenate at 20 µgLane 3 : SH-SY5Y (Human neuroblastoma cell line) whole cell lysate at 20 µgLane 4 : Mouse brain homogenate at 20 µgSecondaryGoat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
SNAP25 was immunoprecipitated using 0.5mg Rat Spinal Cord tissue lysate, 5µg of Rabbit polyclonal to SNAP25 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Rat Spinal Cord tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab41455.Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.Band: 26kDa; SNAP25
Product References
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