All lanes : Anti-SNAP25 antibody [4C6] (ab119028) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SNAP25 cDNALysates/proteins at 5 µg per lane.
ab119028, at 1/100 dilution, staining SNAP25 in COS7 cells transiently transfected with pCMV6-ENTRY SNAP25 by Immunofluorescence.
HEK293T cells transfected with either a SNAP25 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained using ab119028 at 1/100 dilution and then analyzed by flow cytometry.