Anti-SNF2H antibody
| Name | Anti-SNF2H antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab72499 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF IHC-P WB IP |
| Species Reactivities | Mouse, Human, Rat, Rabbit, Horse, Guinea Pig, Bovine, Dog, Pig, Chimpanzee, Monkey, Ape, Orangutan |
| Antigen | Synthetic peptide corresponding to a region between residues 50 and 100 of human SNF2H (NP_003592 |
| Description | Rabbit Polyclonal |
| Gene | SMARCA5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-SNF2H antibody (ab72499) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µg
Detection of SNF2H by Western Blot of Immunprecipitate. ab72499 at 1µg/ml staining SNF2H in HeLa whole cell lysate immunoprecipitated using ab72499 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
ICC/IF image of ab72499 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72499, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab72499 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72499, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Snf2h-mediated chromatin organization and histone H1 dynamics govern cerebellar - Snf2h-mediated chromatin organization and histone H1 dynamics govern cerebellar
Alvarez-Saavedra M, De Repentigny Y, Lagali PS, Raghu Ram EV, Yan K, Hashem E, Ivanochko D, Huh MS, Yang D, Mears AJ, Todd MA, Corcoran CP, Bassett EA, Tokarew NJ, Kokavec J, Majumder R, Ioshikhes I, Wallace VA, Kothary R, Meshorer E, Stopka T, Skoultchi AI, Picketts DJ. Nat Commun. 2014 Jun 20;5:4181.
Functional coupling between HIV-1 integrase and the SWI/SNF chromatin remodeling - Functional coupling between HIV-1 integrase and the SWI/SNF chromatin remodeling
Lesbats P, Botbol Y, Chevereau G, Vaillant C, Calmels C, Arneodo A, Andreola ML, Lavigne M, Parissi V. PLoS Pathog. 2011 Feb 10;7(2):e1001280.