SMARCA5

Gene Symbol SMARCA5
Entrez Gene 8467
Alt Symbol ISWI, SNF2H, WCRF135, hISWI, hSNF2H
Species Human
Gene Type protein-coding
Description SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
Other Description SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5|sucrose nonfermenting protein 2 homolog|sucrose nonfermenting-like 5
Swissprots O60264
Accessions AAY40920 AAY40965 EAX05071 O60264 AB010882 BAA25173 AF086544 AK123439 AK302841 BAG64032 AK308424 AK316444 BAH14815 AL157495 AU132439 BC023144 AAH23144 JF432501 ADZ15718 XM_011532361 XP_011530663 NM_003601 NP_003592
Function Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar r
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00624, ECO:0000269|PubMed:12434153}.
Tissue Specificity Ubiquitously expressed.

SNF2H Antibody - 13453 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using SNF2H Antibody.

hSNF2H (D-10) - sc-365727 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
hSNF2H (D-10): sc-365727. Western blot analysis of hSNF2H expression in non-transfected: sc-117752 (A) and human hSNF2H transfected: sc-113724 (B) 293T whole cell lysates.

hSNF2H (C-16) - sc-8760 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
hSNF2H (C-16): sc-8760. Western blot analysis of hSNF2H expression in K-562 (A) and Jurkat (B) whole cell lysates.

hSNF2H (H-300) - sc-13054 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
hSNF2H (H-300): sc-13054. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human ovary tumor showing nuclear localization.

hSNF2H (N-17) - sc-8759 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
hSNF2H (N-17): sc-8759. Western blot analysis of hSNF2H expression in non-transfected 293T: sc-117752 (A), human hSNF2H transfected 293T: sc-113724 (B) and K-562 (C) whole cell lysates.

Anti-SNF2H antibody - ab72499 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SNF2H antibody (ab72499) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µg

Anti-SNF2H antibody - ab185725 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach cancer tissue labelling SNF2H with ab185725 at 1/200. Magnification: 400x.

Anti-SNF2H antibody - ab168653 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-SNF2H antibody (ab168653) at 1 µg/mlLane 1 : SNF2H transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysate Lysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-SNF2H antibody - ab95203 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SNF2H antibody - ab76459 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-SNF2H antibody (ab76459) at 1 µg/ml + Recombinant tagged SNF2H fragment at 0.1 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Anti-SNF2H antibody - ab123662 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-SNF2H antibody (ab123662) at 1/100 dilution + SK-BR-3 cell line lysates at 35 µg

Anti-SNF2H antibody [3.25(2)] - ab33747 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3.25(2)
ICC/IF image of ab33747 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33747, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10min) HEK293 cells, HepG2 cells at 5ug/ml and in 100% Methanol fixed (5 min) HeLa cells, HEK293 cells, HepG2 cells, and MCF-7 cells at 5µg/ml.

Anti-SNF2H antibody [EPR7673(2)] - ab183730 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7673(2)
Immunofluorescence analysis of HeLa cells (fixative -20℃ Acetone) labeling SNF2H with ab183730 at a 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution. Right panel counterstain with Dapi.

Anti-SNF2H antibody - ChIP Grade - ab3749 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMARCA5 / SNF2H Antibody (aa946-975, Biotin) - LS-C215292 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMARCA5 / SNF2H Antibody (aa946-975, FITC) - LS-C215293 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMARCA5 / SNF2H Antibody (aa946-975, Azide-free, HRP) - LS-C215294 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMARCA5 / SNF2H Antibody (aa946-975, PE) - LS-C215295 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMARCA5 / SNF2H Antibody (aa62-91, AP) - LS-C215296 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMARCA5 / SNF2H Antibody (aa62-91) - LS-C165308 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ISWI Antibody western blot of SK-BR-3 cell line lysates (35 ug/lane). The ISWI antibody detected the ISWI protein (arrow).

Anti-SMARCA5 / SNF2H Antibody (aa62-91, APC) - LS-C215297 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMARCA5 / SNF2H Antibody (N-Terminus) - LS-C80202 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NTERA2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6%-18%.

Anti-SMARCA5 / SNF2H Antibody (aa62-91, Biotin) - LS-C215298 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMARCA5 / SNF2H Antibody (aa51-100) - LS-C79941 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.

Anti-SMARCA5 / SNF2H Antibody (aa62-91, FITC) - LS-C215299 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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