Anti-SNF2H antibody [3.25(2)]

• Supplier: Abcam
• Catalog: ab33747
• Prices: $378.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone: 3.25(2)
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Recombinant fusion protein SNF2H
• Description: Mouse Monoclonal
• Gene: SMARCA5
• Conjugate: Unconjugated

Product images

ICC/IF image of ab33747 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33747, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10min) HEK293 cells, HepG2 cells at 5ug/ml and in 100% Methanol fixed (5 min) HeLa cells, HEK293 cells, HepG2 cells, and MCF-7 cells at 5µg/ml.

All lanes : Anti-SNF2H antibody [3.25(2)] (ab33747) at 10 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Overlay histogram showing HeLa cells stained with ab33747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33747, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Product References

NuMA promotes homologous recombination repair by regulating the accumulation of - NuMA promotes homologous recombination repair by regulating the accumulation of

Vidi PA, Liu J, Salles D, Jayaraman S, Dorfman G, Gray M, Abad P, Moghe PV, Irudayaraj JM, Wiesmuller L, Lelievre SA. Nucleic Acids Res. 2014 Jun;42(10):6365-79.