Anti-SNF2H antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab3749
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IP ICC/IF ICC/IF ChIP WB
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide derived from residues 1000 to the C-terminus of Human SNF2H
• Description: Rabbit Polyclonal
• Gene: SMARCA5
• Conjugate: Unconjugated

Product images

  ab3749 works in ChIP, as shown by the detection of an increase in the recruitment of SNF2H to the estrogen-responsive pS2 promoter.  Sonicated Chromatin prepared from untreated or 17beta-estradiol (E) treated MCF7 cells was subjected to the ChIP procedure with ab3749 antibody specific to SNF2H. The immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers were designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 2.5 µl of ab3749 and 2x106 cells were used in each ChIP experiment.    

Interphase HeLa cells immunofluorescently labeled with anti-SNF2H ab3749 (1/500). This image shows a strong and predominantly nuclear localisation of SNF2H. Secondary antibody used: polyclonal goat anti-rabbit antibody (1/200) conjugated to Cy3.See Abreview

Product References

Epigenetic control of SPI1 gene by CTCF and ISWI ATPase SMARCA5. - Epigenetic control of SPI1 gene by CTCF and ISWI ATPase SMARCA5.

Dluhosova M, Curik N, Vargova J, Jonasova A, Zikmund T, Stopka T. PLoS One. 2014 Feb 3;9(2):e87448.

NuMA promotes homologous recombination repair by regulating the accumulation of - NuMA promotes homologous recombination repair by regulating the accumulation of

Vidi PA, Liu J, Salles D, Jayaraman S, Dorfman G, Gray M, Abad P, Moghe PV, Irudayaraj JM, Wiesmuller L, Lelievre SA. Nucleic Acids Res. 2014 Jun;42(10):6365-79.

Glycogen synthase kinase (GSK) 3beta phosphorylates and protects nuclear myosin - Glycogen synthase kinase (GSK) 3beta phosphorylates and protects nuclear myosin

Sarshad AA, Corcoran M, Al-Muzzaini B, Borgonovo-Brandter L, Von Euler A, Lamont D, Visa N, Percipalle P. PLoS Genet. 2014 Jun 5;10(6):e1004390.

Neurospora WC-1 recruits SWI/SNF to remodel frequency and initiate a circadian - Neurospora WC-1 recruits SWI/SNF to remodel frequency and initiate a circadian

Wang B, Kettenbach AN, Gerber SA, Loros JJ, Dunlap JC. PLoS Genet. 2014 Sep 25;10(9):e1004599.

Nuclear myosin 1c facilitates the chromatin modifications required to activate - Nuclear myosin 1c facilitates the chromatin modifications required to activate

Sarshad A, Sadeghifar F, Louvet E, Mori R, Bohm S, Al-Muzzaini B, Vintermist A, Fomproix N, Ostlund AK, Percipalle P. PLoS Genet. 2013 Mar;9(3):e1003397.

Identification of proteins at active, stalled, and collapsed replication forks - Identification of proteins at active, stalled, and collapsed replication forks

Sirbu BM, McDonald WH, Dungrawala H, Badu-Nkansah A, Kavanaugh GM, Chen Y, Tabb DL, Cortez D. J Biol Chem. 2013 Nov 1;288(44):31458-67.

The miR-99 family regulates the DNA damage response through its target SNF2H. - The miR-99 family regulates the DNA damage response through its target SNF2H.

Mueller AC, Sun D, Dutta A. Oncogene. 2013 Feb 28;32(9):1164-72.

Epigenetic transcriptional regulation of the growth arrest-specific gene 1 (Gas1) - Epigenetic transcriptional regulation of the growth arrest-specific gene 1 (Gas1)

Sacilotto N, Espert A, Castillo J, Franco L, Lopez-Rodas G. PLoS One. 2011;6(8):e23318.

Chromatin remodeling by imitation switch (ISWI) class ATP-dependent remodelers is - Chromatin remodeling by imitation switch (ISWI) class ATP-dependent remodelers is

Goldman JA, Garlick JD, Kingston RE. J Biol Chem. 2010 Feb 12;285(7):4645-51.

Multiple epigenetic modifiers induce aggressive viral extinction in - Multiple epigenetic modifiers induce aggressive viral extinction in

Golding MC, Zhang L, Mann MR. Cell Stem Cell. 2010 May 7;6(5):457-67.