Anti-SNRPD2 antibody [EPR16762]

• Supplier: Abcam
• Catalog: ab198296
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16762
• Applications: WB ICC/IF ICC/IF IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SNRPD2 aa 50 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: SNRPD2
• Conjugate: Unconjugated

Product images

All lanes : Anti-SNRPD2 antibody [EPR16762] (ab198296) at 1/20000 dilutionLane 1 : HepG2 cell lysateLane 2 : MCF7 cell lysateLane 3 : A549 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique

Lanes 1 - 6 : Anti-SNRPD2 antibody [EPR16762] (ab198296) at 1/2000 dilutionLane 7 : Anti-SNRPD2 antibody [EPR16762] (ab198296) at 1/20000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse spleen lysateLane 3 : Rat brain lysateLane 4 : Rat spleen lysateLane 5 : C6 cell lysateLane 6 : RAW 264.7 cell lysateLane 7 : PC12 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SNRPD2 with ab198296 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Human kidney tissue is observed. Counter stained with Hematoxylin.Negative control Using PBS instead of primary antbody, secondary ab is Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling SNRPD2 with ab198296 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Rat kidney tissue is observed. Counter stained with Hematoxylin.Negative control Using PBS instead of primary antbody, secondary ab is Goat Anti-Rabbit IgG H&L (HRP)

NRPD2 protein was immunoprecipitation from 1mg of MCF-7 (Human breast adenocarcinoma) whole cell lysate with ab198296 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab198296 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution. Lane 1: Input, MCF-7 (Human breast adenocarcinoma) whole cell lysate, 10ug. Lane 2: IP of NRPD2 from MCF-7 (Human breast adenocarcinoma) whole cell lysate. Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab198296 in MCF-7 (Human breast adenocarcinoma) whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.