Anti-SNX27 antibody [1C6]
| Name | Anti-SNX27 antibody [1C6] |
|---|---|
| Supplier | Abcam |
| Catalog | ab77799 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 1C6 |
| Applications | FC WB IP ICC/IF ICC/IF |
| Species Reactivities | Human, Monkey |
| Antigen | Fusion protein of N-terminal Human SNX27 |
| Description | Mouse Monoclonal |
| Gene | SNX27 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-SNX27 antibody [1C6] (ab77799) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/3036_SNX27-Primary-antibodies-ab77799-3.jpg)
All lanes : Anti-SNX27 antibody [1C6] (ab77799) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab77799 (1/500) detecting SNX27 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.See Abreview
![Overlay histogram showing Jurkat cells stained with ab77799 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77799, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/3038_SNX27-Primary-antibodies-ab77799-7.jpg)
Overlay histogram showing Jurkat cells stained with ab77799 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77799, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
SNX27 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Mouse monoclonal to SNX27 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab77799.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.Band: 61kDa: SNX27.
Product References
Subcellular sorting of the G-protein coupled mouse somatostatin receptor 5 by a - Subcellular sorting of the G-protein coupled mouse somatostatin receptor 5 by a
Bauch C, Koliwer J, Buck F, Honck HH, Kreienkamp HJ. PLoS One. 2014 Feb 11;9(2):e88529.
RME-8 coordinates the activity of the WASH complex with the function of the - RME-8 coordinates the activity of the WASH complex with the function of the
Freeman CL, Hesketh G, Seaman MN. J Cell Sci. 2014 May 1;127(Pt 9):2053-70.
A unique PDZ domain and arrestin-like fold interaction reveals mechanistic - A unique PDZ domain and arrestin-like fold interaction reveals mechanistic
Gallon M, Clairfeuille T, Steinberg F, Mas C, Ghai R, Sessions RB, Teasdale RD, Collins BM, Cullen PJ. Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):E3604-13. doi:
A global analysis of SNX27-retromer assembly and cargo specificity reveals a - A global analysis of SNX27-retromer assembly and cargo specificity reveals a
Steinberg F, Gallon M, Winfield M, Thomas EC, Bell AJ, Heesom KJ, Tavare JM, Cullen PJ. Nat Cell Biol. 2013 May;15(5):461-71.
New sorting nexin (SNX27) and NHERF specifically interact with the 5-HT4a - New sorting nexin (SNX27) and NHERF specifically interact with the 5-HT4a
Joubert L, Hanson B, Barthet G, Sebben M, Claeysen S, Hong W, Marin P, Dumuis A, Bockaert J. J Cell Sci. 2004 Oct 15;117(Pt 22):5367-79. Epub 2004 Oct 5.