All lanes : Anti-SNX5 antibody [EPR14358-10] (ab183719) at 1/1000 dilutionLane 1 : Jurkat whole cell lysateLane 2 : 293T whole cell lysateLane 3 : HeLa whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Lysate from 293T cells was immunoprecipitated with ab183719 at a 1/50 dilution. For the subsequent blot, ab183719 was used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Lane 2: TBS instead of 293T lysates. Blocking/ Dilution buffer: 5% NFDM/TBST.