Anti-SOCS1 antibody
| Name | Anti-SOCS1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab62584 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic 15 amino acid peptide from near the carboxy terminus of human SOCS1 |
| Description | Rabbit Polyclonal |
| Gene | SOCS1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-SOCS1 antibody (ab62584) at 1 µg/mlLane 2 : Anti-SOCS1 antibody (ab62584) at 2 µg/mlLane 3 : Anti-SOCS1 antibody (ab62584) at 4 µg/mlLane 1 : Human spleen cell lysateLane 2 : Human spleen cell lysateLane 3 : Human spleen cell lysateLysates/proteins at 15 µg per lane.
ab62584 (4µg/ml) staining SOCS1 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasmic and nuclei of macrophages and some pneumocytes. There was also cytoplasmic and nuclei staining of epithelium cells of the bronchioles.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ICC/IF image of ab62584 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62584, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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