Flow cytometric analysis of SOCS1 expression in WiDr cells labelled with ab135718 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescence analysis of SOCS1 expression in 293 cells labelled with ab135718 at 1/10 dilution. This was followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemical analysis of SOCS1 expression in paraffin embedded, formalin fixed Human colon carcinoma tissue labelled with ab135718 at 1/50 dilution. This was followed by incubation with a peroxidase conjugated secondary antibody and DAB staining.
Anti-SOCS1 antibody - N-terminal (ab135718) at 1/50 dilution + Mouse kidney tissue lysates at 35 µg