Anti-SOCS3 antibody
| Name | Anti-SOCS3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab16030 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF FC WB IHC-F IHC-P |
| Species Reactivities | Mouse, Rat, Human, Chicken, Bovine, Dog |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human SOCS3 |
| Blocking Peptide | Human SOCS3 peptide |
| Description | Rabbit Polyclonal |
| Gene | SOCS3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-SOCS3 antibody (ab16030) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/mlLane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/mlLane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/mlLane 8 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate (ab52252) with Human SOCS3 peptide (ab16199) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.Observed
ICC/IF image of ab16030 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab16030, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Image courtesy of Human Protein Atlasab16030 staining SOCS3 in human bone marrow showing strong staining within the bone marrow. Paraffin embedded human bone marrow was incubated with ab16030 (1/100) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH6.ab16030 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
ab16030 staining SOCS3 in Human pancreatic tumour tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/300 in PBS) for 8 hours at 4°C. An Alexa Fluor®555-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were stained by DAPI.See Abreview
All lanes : Anti-SOCS3 antibody (ab16030) at 1 µg/mlLane 1 : Untreated Human peripheral blood mononuclear cell lysateLane 2 : Human peripheral blood mononuclear cell lysate (treated with aCD3/28)Lane 3 : Human peripheral blood mononuclear cell lysate (treated with IFNγ)Lane 4 : SOCS3 proteinLysates/proteins at 15 µg per lane.SecondaryHRP-conjugated goat anti-rabbit polyclonal IgG at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
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