All lanes : Anti-SOCS3 antibody ( ab16030 ) at 1 µg/ml Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/ml Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/ml Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/ml Lane 8 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate ( ab52252 ) with Human SOCS3 peptide (ab16199) at 1 µg/ml Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Performed under reducing condit