Anti-Sodium / Hydrogen Exchanger 1 antibody
| Name | Anti-Sodium / Hydrogen Exchanger 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58304 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: VLRSHGLQLS PTASTIRSSE PPRERSIGDV TTAPPEVTPE SRPVNHSVTD HGMKPRKAFP VLGIDYTHVR TPFEISLWIL LACLMKIGFH VIPTISSIVP , corresponding to amino acids 31-130 of Human Sodium/ Hydrogen Exchanger 1 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | SLC9A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Sodium / Hydrogen Exchanger 1 antibody (ab58304) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.
Western blot against tagged recombinant protein immunogen using ab58304 Sodium / Hydrogen Exchanger 1 antibody at 1ug/ml.
![Overlay histogram showing HEK293 cells stained with ab58304 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58304, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed HEK293 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/3273_Sodium-Hydrogen-Exchanger-1-Primary-antibodies-ab58304-2.jpg)
Overlay histogram showing HEK293 cells stained with ab58304 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58304, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed HEK293 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.