Anti-Sodium Iodide Symporter antibody [SPM186]
| Name | Anti-Sodium Iodide Symporter antibody [SPM186] |
|---|---|
| Supplier | Abcam |
| Catalog | ab17795 |
| Prices | $400.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | SPM186 |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Rat, Human |
| Antigen | Synthetic peptide: WTPCV GHDGGRDQQE TNL , corresponding to amino acids 625-643 of Human Sodium Iodide Symporter |
| Description | Mouse Monoclonal |
| Gene | SLC5A5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab17795 staining Sodium Iodide Symporter in Rat thyrocytes (Pccl3) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% saponin and blocked with 5% BSA for 1 hour at 37°C. Samples were incubated with primary antibody (1/50 in PBS + 1% BSA + 0.1% saponin) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.See Abreview
ab17795 staining Sodium Iodide Symporter in Human thyrocytes (primary culture) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% saponin in PBS and blocked with 5% BSA + 0.1% saponin in PBS for 1 hour at 37°C. Samples were incubated with primary antibody (1/50 in PBS + 1% BSA + 0.1% saponin) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.See Abreview
ab17795 at 1/50 staining sodium iodide symporter from human thyroid by immunohistochemistry (formalin-fixed, paraffin embedded).
![Anti-Sodium Iodide Symporter antibody [SPM186] (ab17795) at 1 µg/ml + Breast (Human) Tissue Lysate - normal tissue (ab30090) at 10 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/3286_Sodium-Iodide-Symporter-Primary-antibodies-ab17795-2.jpg)
Anti-Sodium Iodide Symporter antibody [SPM186] (ab17795) at 1 µg/ml + Breast (Human) Tissue Lysate - normal tissue (ab30090) at 10 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Overlay histogram showing MCF7 cells stained with ab17795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17795, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/3287_Sodium-Iodide-Symporter-Primary-antibodies-ab17795-3.jpg)
Overlay histogram showing MCF7 cells stained with ab17795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17795, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
IHC Profiler: an open source plugin for the quantitative evaluation and automated - IHC Profiler: an open source plugin for the quantitative evaluation and automated
Varghese F, Bukhari AB, Malhotra R, De A. PLoS One. 2014 May 6;9(5):e96801.
Quantitative immunohistochemical analysis reveals association between sodium - Quantitative immunohistochemical analysis reveals association between sodium
Chatterjee S, Malhotra R, Varghese F, Bukhari AB, Patil A, Budrukkar A, Parmar V, Gupta S, De A. PLoS One. 2013;8(1):e54055.
Noninvasive visualization of microRNA-16 in the chemoresistance of gastric cancer - Noninvasive visualization of microRNA-16 in the chemoresistance of gastric cancer
Wang F, Song X, Li X, Xin J, Wang S, Yang W, Wang J, Wu K, Chen X, Liang J, Tian J, Cao F. PLoS One. 2013 Apr 17;8(4):e61792.