Anti-Somatostatin Receptor 5 antibody
| Name | Anti-Somatostatin Receptor 5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28618 |
| Prices | $384.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide corresponding to Human Somatostatin Receptor 5 aa 344-364 |
| Description | Rabbit Polyclonal |
| Gene | SSTR5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Somatostatin Receptor 5 antibody (ab28618) at 1 µg/ml + Brain: pituitary gland (Human) Whole Cell Lysate - adult normal tissue (ab29749) at 10 µgSecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 42 kDa (why is the actual band size different from the predicted?)Additional bands at : 15 kDa,20 kDa,73 kDa. We are unsure as to the identity of these extra bands.Exposure time : 8 minutesSomatostatin Receptor 5 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ICC/IF image of ab28618 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28618, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Expression of Ki-67, PTTG1, FGFR4, and SSTR 2, 3, and 5 in nonfunctioning - Expression of Ki-67, PTTG1, FGFR4, and SSTR 2, 3, and 5 in nonfunctioning
Ramirez C, Cheng S, Vargas G, Asa SL, Ezzat S, Gonzalez B, Cabrera L, Guinto G, Mercado M. J Clin Endocrinol Metab. 2012 May;97(5):1745-51.
Overexpression and functional relevance of somatostatin receptor-1, -2, and -5 in - Overexpression and functional relevance of somatostatin receptor-1, -2, and -5 in
Fasciani A, Quilici P, Biscaldi E, Flamini M, Fioravanti A, Orlandi P, Oliviero J, Repetti F, Bandelloni R, Danesi R, Simoncini T, Bocci G. J Clin Endocrinol Metab. 2010 Dec;95(12):5315-9.
Increased fatigue resistance linked to Ca2+-stimulated mitochondrial biogenesis - Increased fatigue resistance linked to Ca2+-stimulated mitochondrial biogenesis
Bruton JD, Aydin J, Yamada T, Shabalina IG, Ivarsson N, Zhang SJ, Wada M, Tavi P, Nedergaard J, Katz A, Westerblad H. J Physiol. 2010 Nov 1;588(Pt 21):4275-88.
Somatostatin receptor tissue distribution in lung neuroendocrine tumours: a - Somatostatin receptor tissue distribution in lung neuroendocrine tumours: a
Righi L, Volante M, Tavaglione V, Bille A, Daniele L, Angusti T, Inzani F, Pelosi G, Rindi G, Papotti M. Ann Oncol. 2010 Mar;21(3):548-55.