![All lanes : Anti-SorLA antibody [3B6B11] (ab63336) at 1/500 dilutionLane 1 : Molecular Weight MarkersLane 2 : truncated recombinant SORL1-MBP immunizing peptide](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/3493_ab63336wb.jpg)
All lanes : Anti-SorLA antibody [3B6B11] (ab63336) at 1/500 dilutionLane 1 : Molecular Weight MarkersLane 2 : truncated recombinant SORL1-MBP immunizing peptide
ab63336 at 1/500 dilution with DAB staining on normal human cerebrum tissue; paraffin embedded. Showing cytoplasma location.
ab63336 at 1/1000 dilution staining SorLA in PANC1 cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated anti-mouse IgG was used as secondary. Actin filaments were labeled red with Alexa Fluor-555 phalloidin whilst nuclei were stained blue with DRAQ5 fluorescent DNA dye.
![Overlay histogram showing SH-SY5Y cells stained with ab63336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab63336, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/3496_SorLA-Primary-antibodies-ab63336-2.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab63336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab63336, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.