Anti-Sortilin antibody
| Name | Anti-Sortilin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab16640 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF FC IP ICC/IF IHC-F |
| Species Reactivities | Mouse, Rat, Bovine, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human Sortilin |
| Blocking Peptide | Human Sortilin peptide |
| Description | Rabbit Polyclonal |
| Gene | SORT1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescent staining for Sortilin in rat cortical neurons using Rabbit polyclonal to Sortilin (ab16640). The staining is cytoplasmic and punctate of many cells such as cortical neurons. The image was taken with a X20 objective.Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT and then cut on cryostat (30µm coronal sections). IHC was perfored in free floating with fixed tissues (rat brain sections). Primary antibody was incubated overnight at 1/3000 at room temperature. Secondary antibody Alexa fluor 488 1/1000 for 2 hours at room temperature.
ab16640 staining cultured mouse astrocytes by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured cells were then stained with ab16640 at 1/1000 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. Sortilin expressed in the cytosol mainly in trans-golgi compartments.See Abreview
Lane 1 : MarkerLanes 2 - 4 : Anti-Sortilin antibody (ab16640) at 1 µg/mlLane 1 : As aboveLane 2 : Human Brain Tissue Lysate at 20 µg Lane 3 : Mouse Brain at 20 µg Lane 4 : Brain (Rat) Whole Cell Lysate - normal tissue at 20 µgSecondaryLanes 2 - 4 : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab16640 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16640, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Inhibition of neuronal apoptosis and axonal regression ameliorates sympathetic - Inhibition of neuronal apoptosis and axonal regression ameliorates sympathetic
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Sortilin expression is essential for pro-nerve growth factor-induced apoptosis of - Sortilin expression is essential for pro-nerve growth factor-induced apoptosis of
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Fish oil and fenofibrate prevented phosphorylation-dependent hepatic sortilin 1 - Fish oil and fenofibrate prevented phosphorylation-dependent hepatic sortilin 1
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Depletion of FOXP3+ regulatory T cells promotes hypercholesterolemia and - Depletion of FOXP3+ regulatory T cells promotes hypercholesterolemia and
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Saturated fatty acids activate ERK signaling to downregulate hepatic sortilin 1 - Saturated fatty acids activate ERK signaling to downregulate hepatic sortilin 1
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The neurotrophic properties of progranulin depend on the granulin E domain but do - The neurotrophic properties of progranulin depend on the granulin E domain but do
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Targeted manipulation of the sortilin-progranulin axis rescues progranulin - Targeted manipulation of the sortilin-progranulin axis rescues progranulin
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Age-dependent alterations in nerve growth factor (NGF)-related proteins, - Age-dependent alterations in nerve growth factor (NGF)-related proteins,
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Precursor of brain-derived neurotrophic factor (proBDNF) forms a complex with - Precursor of brain-derived neurotrophic factor (proBDNF) forms a complex with
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