All lanes : Anti-SOX17 antibody (ab99370) at 1 µg/mlLane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 3 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 4 : LNCaP human prostate carcinoma cell line lysate Whole Cell LysateLane 5 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell LysateLane 6 : DU 145 (Human prostate carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.