All lanes : Anti-SOX9 (phospho S181) antibody (ab68610) at 1 µg/mlLane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissueLane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissue with Modified blocking peptide at 1 µg/mlLane 4 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889) with Modified blocking peptide at 1 µg/mlLane 5 : Bone Marrow (Human) Tissue Lysate - adult normal tissue with Non - Modified blocking peptide at 1 µg/mlLane 6 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889) with Non - Modified blocking peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.