SOX9

Gene Symbol SOX9
Entrez Gene 6662
Alt Symbol CMD1, CMPD1, SRA1
Species Human
Gene Type protein-coding
Description SRY (sex determining region Y)-box 9
Other Description SRY (sex-determining region Y)-box 9 protein|SRY-related HMG-box, gene 9|transcription factor SOX-9
Swissprots P48436 Q53Y80
Accessions AAB32870 AGZ62956 AGZ62957 AGZ62958 AGZ62959 AGZ62960 AGZ62962 AGZ62963 AGZ62964 AGZ62965 AGZ62966 AGZ62967 AGZ62968 BAN66330 CAI61686 EAW89102 P48436 AK295455 BAG58389 BC007951 AAH07951 BC018276 BC056420 AAH56420 BT006875 AAP35521 Z46629 CAA86598 NM_000346 NP_000337
Function Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00267}.
Top Pathways cAMP signaling pathway

Sox9 Antibody - 14366 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from DLD-1 and U-87 MG cells using Sox9 Antibody.

Sox-9 (C-20) - sc-17341 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Sox-9 (C-20): sc-17341. Western blot analysis of Sox-9 expression in SW480 nuclear extract.

Sox-9 (E-9) - sc-166505 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Sox-9 (E-9): sc-166505. Western blot analysis of Sox-9 expression in non-transfected: sc-117752 (A) and human Sox-9 transfected: sc-116634 (B) 293T whole cell lysates.

Sox-9 (H-90) - sc-20095 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Sox-9 (H-90): sc-20095. Immunofluorescence staining of methanol-fixed SW480 cells showing nuclear localization.

Sox-9 (P-20) - sc-17340 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Sox-9 (P-20): sc-17340. Western blot analysis of Sox-9 expression in non-transfected: sc-117752 (A) and human Sox-9 transfected: sc-116634 (B) 293T whole cell lysates.

Sox-10 (G-11) - sc-374170 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Sox-10 (G-11): sc-374170. Western blot analysis of Sox-10 expression in non-transfected: sc-117752 (A) and human Sox-10 transfected: sc-176025 (B) 293T whole cell lysates.

Sox-10 (H-2) - sc-271163 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Sox-10 (H-2): sc-271163. Western blot analysis of Sox-10 expression in non-transfected: sc-117752 (A) and human Sox-10 transfected: sc-176025 (B) 293T whole cell lysates.

Anti-SOX9 (phospho S181) antibody - ab68610 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SOX9 (phospho S181) antibody (ab68610) at 1 µg/mlLane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissueLane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissue with Modified blocking peptide at 1 µg/mlLane 4 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889) with Modified blocking peptide at 1 µg/mlLane 5 : Bone Marrow (Human) Tissue Lysate - adult normal tissue with Non - Modified blocking peptide at 1 µg/mlLane 6 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889) with Non - Modified blocking peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-SOX9 antibody - ab26414 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SOX9 antibody (ab26414) at 2 µg/mlLane 1 : Neuroectoderm (differentiated from human embryonic stem cells) Day 5 - Whole Cell Lysate Lane 2 : Neuroectoderm (differentiated from human embryonic stem cells) Day 10 - Whole Cell Lysate Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lane 5 : Mouse Fetus (14 Day Old) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-SOX9 antibody - ab59265 from Abcam

Prices $391.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
SOX9 was immunoprecipitated using 0.5mg SW480 whole cell extract, 5µg of Rabbit polyclonal to SOX9 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, SW480 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab59265.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 56kDa; SOX9

Anti-SOX9 antibody - ab118892 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab118892 at 10 µg/ml staining SOX9 in Formalin-fixed, Paraffin-embedded Human Testis tissue by Immunohistochemistry.

Anti-SOX9 antibody - ab58191 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HepG2 cells stained with ab58191 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58191, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-SOX9 antibody - ab36748 from Abcam

Prices $378.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
Human testis stained with Anti-SOX9 (ab36748) at 1/50 dilution by Immunohistochemistry (FFPE-sections) for 30 min at room temperature.

Anti-SOX9 antibody - ab41833 from Abcam

Prices $376.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
Ab41833 staining human SOX9 in human testis by immunohistochemistry using formalin fixed, paraffin embedded tissue.

Anti-SOX9 antibody [3C10] - ab76997 from Abcam

Prices $392.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3C10
Anti-SOX9 antibody [3C10] (ab76997) at 5 µg/ml + immunogen at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Anti-SOX9 antibody [EPR12755] - ab182579 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12755
All lanes : Anti-SOX9 antibody [EPR12755] (ab182579) at 1/10000 dilutionLane 1 : SW480 cell lysateLane 2 : Caco 2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-SOX9 antibody [EPR12755] (Alexa Fluor® 488) - ab202686 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12755
ab202686 staining SOX9 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202686 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-SOX9 antibody [EPR12755-50] - C-terminal - ab184547 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12755-50
All lanes : Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : SW480 cell lysateLane 3 : Caco2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-SOX9 antibody [EPR14335] - ab185230 from Abcam

Prices $99.00, $384.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14335
Anti-SOX9 antibody [EPR14335] (ab185230) at 1/20000 dilution + SW480 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-SOX9 antibody [EPR14335] (Alexa Fluor® 647) - ab196184 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14335
ab196184 staining SOX9 in SW480 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196184 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-SOX9 antibody [EPR14335] (HRP) - ab195550 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14335
IHC image of SOX9 staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195550 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-SOX9 antibody [EPR14335-78] - ab185966 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14335-78
Anti-SOX9 antibody [EPR14335-78] (ab185966) at 1/5000 dilution + PC3 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-SOX9 antibody, prediluted - ab41832 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Ab41832 staining human SOX9 in human testis by immunohistochemistry using formalin fixed, paraffin embedded tissue.

Anti-SOX9 antibody - Carboxyterminal end - ab135098 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-SOX9 antibody - Carboxyterminal end (ab135098) at 1/500 dilution + HepG2 whole cell extracts

Anti-SOX9 Antibody (clone 3C10) IHC-plus™ - LS-B4420 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3C10
Anti-SOX9 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4420 concentration 5 ug/ml.
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