Anti-SOX9 antibody
| Name | Anti-SOX9 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab26414 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IHC-P |
| Species Reactivities | Mouse, Rat, Human, Pig, Bovine, Dog, Marmoset |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SOX9 |
| Description | Rabbit Polyclonal |
| Gene | SOX9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-SOX9 antibody (ab26414) at 2 µg/mlLane 1 : Neuroectoderm (differentiated from human embryonic stem cells) Day 5 - Whole Cell Lysate Lane 2 : Neuroectoderm (differentiated from human embryonic stem cells) Day 10 - Whole Cell Lysate Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lane 5 : Mouse Fetus (14 Day Old) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
Anti-SOX9 antibody (ab26414) at 1 µg/ml + Mouse Fetus (14 Day Old) Tissue Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC-P image of SOX9 (ab26414) on E11.5 mouse embryo sections. The sections were fixed in paraformaldehyde and underwent heat mediated antigen retrieval using Tris/EGTA (pH9). The sections were then blocked in 1% BSA solution for 30 mins at 20°C. In these images ab26414 can be seen staining sections showing spinal cord and developing cartilage (top row), developing cartilage in vertebrae (bottom row).See Abreview
Standard Curve for SOX9 ; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to SOX9 (ab58191) at 5µg/ml and Detector Antibody Rabbit polyclonal to SOX9 (ab26414) at 0.1µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin embedded mouse spleen tissue, staining SOX9 with ab26414.Tissue was fixed with 10% Neutral Buffered Formalin and blocked with 1.5% serum for 45 minutes 21°C; antigen retrieval was by heat mediation in a Tris-EDTA buffer (pH 9). Samples were incubated with primary antibody (0.8 µg/ml in 0.3% Triton X-100 in PBS) for 1 hours at 21°C. An biotin-conjugated goat anti-rabbit polyclonal IgG (7 µg/ml) was used as the secondary antibody. A = Primary antibody B = No primary antibodySee Abreview
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