Anti-SOX9 antibody
| Name | Anti-SOX9 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab59265 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ELISA FC IP |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic non-phosphopeptide derived from human SOX9 around the phosphorylation site of serine 181 (R-K-SP-V-K) |
| Description | Rabbit Polyclonal |
| Gene | SOX9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![SOX9 was immunoprecipitated using 0.5mg SW480 whole cell extract, 5µg of Rabbit polyclonal to SOX9 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, SW480 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab59265.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 56kDa; SOX9](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/3842_ab59265-209885-IPVI004ab5926520m.jpg)
SOX9 was immunoprecipitated using 0.5mg SW480 whole cell extract, 5µg of Rabbit polyclonal to SOX9 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, SW480 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab59265.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 56kDa; SOX9
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59265 at a dilution of 1/50-1/100. Left hand image - without immunising peptide; right hand image - with immunising peptide. Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59265 at a dilution of 1/50-1/100. Left hand image - without immunising peptide; right hand image - with immunising peptide.
All lanes : Anti-SOX9 antibody (ab59265) at 1/500 dilutionLane 1 : extracts from 293 cells, treated with PBS (60mins)Lane 2 : extracts from 293 cells, treated with PBS (60mins) with immunizing non-phosphopeptide
Product References
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Kurth TB, Dell'accio F, Crouch V, Augello A, Sharpe PT, De Bari C. Arthritis Rheum. 2011 May;63(5):1289-300.
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Handorf AM, Li WJ. PLoS One. 2011;6(7):e22887.
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Fanburg-Smith JC, Auerbach A, Marwaha JS, Wang Z, Rushing EJ. Hum Pathol. 2010 May;41(5):653-62.