Anti-SOX9 antibody
| Name | Anti-SOX9 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58191 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ELISA FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 400-510 of Human SOX9 |
| Description | Mouse Monoclonal |
| Gene | SOX9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HepG2 cells stained with ab58191 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58191, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/3849_ab58191-3-ab58191FC.jpg)
Overlay histogram showing HepG2 cells stained with ab58191 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58191, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Standard Curve for SOX9 ; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to SOX9 (ab58191) at 5µg/ml and Detector Antibody Rabbit polyclonal to SOX9 (ab26414) at 0.1µg/ml.
Western blot against tagged recombinant protein immunogen using ab58191 SOX9 antibody at 1ug/ml.