Anti-SOX9 antibody [3C10]
| Name | Anti-SOX9 antibody [3C10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab76997 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | 3C10 |
| Applications | WB ELISA IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment: EQLSPSHYSE QQQHSPQQIA YSPFNLPHYS PSYPPITRSQ YDYTDHQNSS SYYSHAAGQG TGLYSTFTYM NPAQRPMYTP IADTSGVPSI PQTHSPQHWE QPVYTQLTRP, corresponding to amino acids 400-509 of human SOX9 (NP_000337) with a 26 kDa tag Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | SOX9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-SOX9 antibody [3C10] (ab76997) at 5 µg/ml + immunogen at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/3854_WB%2520sox9.jpg)
Anti-SOX9 antibody [3C10] (ab76997) at 5 µg/ml + immunogen at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
![Anti-SOX9 antibody [3C10] (ab76997) at 5 µg/ml + HepG2 cell lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/3855_WB%2520sox9%2520too.jpg)
Anti-SOX9 antibody [3C10] (ab76997) at 5 µg/ml + HepG2 cell lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Immunoperoxidase analysis of SOX9 expression on formalin fixed, paraffin embedded human tonsil tissue using 0.7 µg/ml of ab76997.
Immunofluorescence analysis of SOX9 expression in HepG2 cells, using 10 µg/ml of ab76997.
![Overlay histogram showing HepG2 cells stained with ab76997 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76997, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/3858_SOX9-Primary-antibodies-ab76997-10.jpg)
Overlay histogram showing HepG2 cells stained with ab76997 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76997, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
A genomics approach in determining nanotopographical effects on MSC phenotype. - A genomics approach in determining nanotopographical effects on MSC phenotype.
Tsimbouri PM, Murawski K, Hamilton G, Herzyk P, Oreffo RO, Gadegaard N, Dalby MJ. Biomaterials. 2013 Mar;34(9):2177-84.
SOX9 directly regulates IGFBP-4 in the intestinal epithelium. - SOX9 directly regulates IGFBP-4 in the intestinal epithelium.
Shi Z, Chiang CI, Mistretta TA, Major A, Mori-Akiyama Y. Am J Physiol Gastrointest Liver Physiol. 2013 Jul 1;305(1):G74-83. doi:
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Using nanotopography and metabolomics to identify biochemical effectors of - Using nanotopography and metabolomics to identify biochemical effectors of
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Differential and cooperative actions of Olig1 and Olig2 transcription factors on - Differential and cooperative actions of Olig1 and Olig2 transcription factors on
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Human induced pluripotent stem cells derived from fetal neural stem cells - Human induced pluripotent stem cells derived from fetal neural stem cells
Medvedev SP, Grigor'eva EV, Shevchenko AI, Malakhova AA, Dementyeva EV, Shilov AA, Pokushalov EA, Zaidman AM, Aleksandrova MA, Plotnikov EY, Sukhikh GT, Zakian SM. Stem Cells Dev. 2011 Jun;20(6):1099-112.