All lanes : Anti-SOX9 antibody [EPR12755] (ab182579) at 1/10000 dilutionLane 1 : SW480 cell lysateLane 2 : Caco 2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of Caco 2 cells labeling SOX9 using ab182579 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde.
Immunofluorescence analysis of SW480 cells labeling SOX9 using ab182579 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde.
Detection of SOX9 by Western Blot of Immunprecipitate.Caco 2 cell lysate immunoprecipitated using ab182579 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.