Anti-SOX9 antibody [EPR12755-50] - C-terminal

• Supplier: Abcam
• Catalog: ab184547
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR12755-50
• Applications: FC ICC/IF ICC/IF WB
• Species Reactivities: Human
• Antigen: Recombinant fragment within Human SOX9 aa 400 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: SOX9
• Conjugate: Unconjugated

Product images

All lanes : Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : SW480 cell lysateLane 3 : Caco2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescence analysis of 4% paraformaldehyde-fixed Caco2 cells, labeling SOX9 with ab184547 at 1/500 dilution (red). Alexa Fluor®555-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

Immunofluorescence analysis of 4% paraformaldehyde-fixed HepG2 cells, labeling SOX9 with ab184547 at 1/500 dilution (green). Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

Flow cytometric analysis of HepG2 cells (2% paraformaldehyde-fixed) labeling SOX9 with ab184547 at 1/200 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.