All lanes : Anti-SP100 antibody (ab43151) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 3 : Hodgkin's Lymphoma (Human) Whole Cell Lysate - tumor tissue (ab29923)Lane 4 : Lymphoma (Human) Whole Cell Lysate - tumor tissue (ab30185)Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 6 : ZR-75-1 (Human breast carcinoma cell line) Nuclear lysate (ab14916)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ab43151 staining SP100 in human lymphoma tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with peroxidase for 5 minutes followed by a protein block for 10 minutes at 20°C . Antigen retrieval was by heat mediation in target retrieval solution. Samples were incubated with primary antibody 1/2000 for 45 minutes at 20°C. An HRP-conjugated Goat polycolonal to rabbit IgG was used as secondary antibody.See Abreview