Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labelling SPARC with ab135601 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometric analysis of Widr cells labelling SPARC with ab135601 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC conjugated goat anti-rabbit secondary antibodies were used for the analysis.
Anti-SPARC antibody - C-terminal (ab135601) at 1/50 dilution + Y79 cell lysate at 35 µg
All lanes : Anti-SPARC antibody - C-terminal (ab135601) at 1/50 dilutionLane 1 : 293 cell lysate, non-transfectedLane 2 : 293 cell lysate, transiently transfected with the SPARC geneLysates/proteins at 2 µg per lane.