Anti-Spastin antibody [Sp 3G11/1]

• Supplier: Abcam
• Catalog: ab31850
• Prices: $379.00
• Sizes: 50 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: Sp 3G11/1
• Applications: WB ELISA IHC-P ICC/IF ICC/IF
• Species Reactivities: Rat, Human
• Antigen: Recombinant full length protein (Human)
• Description: Mouse Monoclonal
• Gene: SPAST
• Conjugate: Unconjugated

Product images

Anti-Spastin antibody [Sp 3G11/1] (ab31850) at 1/500 dilution + rat brain synaptosome Observed band size : 52 kDa (why is the actual band size different from the predicted?)

IHC image of Spastin staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31850, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

A novel role for the AAA ATPase spastin as a HOXA10 transcriptional corepressor - A novel role for the AAA ATPase spastin as a HOXA10 transcriptional corepressor

Daftary GS, Tetrault AM, Jorgensen EM, Sarno J, Taylor HS. Mol Endocrinol. 2011 Sep;25(9):1539-49.

Human spastin has multiple microtubule-related functions. - Human spastin has multiple microtubule-related functions.

Salinas S, Carazo-Salas RE, Proukakis C, Cooper JM, Weston AE, Schiavo G, Warner TT. J Neurochem. 2005 Dec;95(5):1411-20. Epub 2005 Oct 7.