SPAST

Gene Symbol SPAST
Entrez Gene 6683
Alt Symbol ADPSP, FSP2, SPG4
Species Human
Gene Type protein-coding
Description spastin
Other Description spastic paraplegia 4 (autosomal dominant; spastin)|spastic paraplegia 4 protein
Swissprots A7E2A7 Q9UPR9 Q9UBP0
Accessions ADP91574 ADP91575 ADP91576 ADP91577 ADP91578 ADP91579 ADP91580 ADP91581 ADP91582 ADP91583 ADP91584 ADP91585 ADP91586 ADP91587 ADP91588 ADP91589 ADP91590 ADP91591 ADP91592 ADP91593 ADP91594 ADP91595 ADP91596 ADP91597 ADP91598 ADP91599 ADP91600 ADP91601 ADP91602 ADP91603 ADP91604 ADP91605 ADP91606 ADP91607 ADP91608 ADP91609 ADP91610 ADP91611 ADP91612 ADP91613 ADP91614 ADP91615 ADP91616 ADP91617 ADP91618 ADP91619 ADP91620 ADP91621 ADP91622 ADP91623 ADP91624 ADP91625 ADP91626 ADP91627 ADP91628 ADP91629 ADP91630 ADP91631 ADP91632 ADP91633 ADP91634 ADP91635 ADP91636 ADP91637 ADP91638 ADP91639 ADP91640 ADP91641 ADP91642 ADP91643 ADP91644 ADP91645 ADP91646 ADP91647 ADP91648 ADP91649 ADP91650 ADP91651 ADP91652 ADP91653 CAB60208 EAX00461 EAX00462 Q9UBP0 AA830062 AB029006 BAA83035 AJ246001 CAB60141 AK304135 BAG65029 BC150260 AAI50261 BG532310 XM_005264516 XP_005264573 XM_011533067 XP_011531369 NM_014946 NP_055761 NM_199436 NP_955468
Function ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis. May also play a role in axon growth and the formation of axonal branches. {ECO:0000269|PubMed:11809724, ECO:0000269|PubMed:15716377, ECO:0000269|PubMed:16219033, ECO:0000269|PubMed:17389232, ECO:0000269|PubMed:19000169, ECO:0000269|PubMed:22637577}.
Subcellular Location Membrane {ECO:0000255|HAMAP-Rule:MF_03021}; Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_03021}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Endoplasmic reticulum. Endosome. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Localization to the centrosome is independent of microtubules. Localizes to the midbody of dividing cells, and this requires CHMP1B. Enriched in the distal axons and branches of postmitotic neurons. Isoform 3 is the main endosomal form.
Tissue Specificity Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. The short isoforms may predominate in brain and spinal cord. {ECO:0000269|PubMed:10610178}.

Spastin (C-3) - sc-374068 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Spastin (C-3): sc-374068. Western blot analysis of Spastin expression in 293T (A) and HeLa (B) whole cell lysates.

Spastin (A-2) - sc-271247 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Spastin (A-2): sc-271247. Western blot analysis of Spastin expression in HeLa whole cell lysate.

Spastin (H-134) - sc-98962 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Spastin (Sp 3G11/1) - sc-53443 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Spastin (Sp 3G11/1): sc-53443. Western blot analysis of Spastin expression in non-transfected 293T: sc-117752 (A), mouse Spastin transfected 293T: sc-123732 (B) and HeLa (C) whole cell lysates.

Spastin (Sp 6C6) - sc-81624 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Spastin (T-15) - sc-49528 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Spastin (T-15): sc-49528. Western blot analysis of Spastin expression in non-transfected: sc-117752 (A) and mouse Spastin transfected: sc-123732 (B) 293T whole cell lysates.

Spastin (D-20) - sc-49525 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Spastin (P-14) - sc-49526 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Spastin antibody - ab101797 from Abcam

Host Rabbit
Clonality Polyclonal
ICC/IF image of ab101797 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101797, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Spastin antibody - ab89449 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-Spastin antibody (ab89449) at 1 µg/ml + Recombinant Spastin Protein (Immunogen) at 0.2 µg

Anti-Spastin antibody - ab35100 from Abcam

Prices $359.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal

Anti-Spastin antibody [Sp 3G11/1] - ab31850 from Abcam

Prices $379.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone Sp 3G11/1
Anti-Spastin antibody [Sp 3G11/1] (ab31850) at 1/500 dilution + rat brain synaptosome Observed band size : 52 kDa (why is the actual band size different from the predicted?)

Anti-Spastin antibody [Sp 6C6] - ab77144 from Abcam

Prices $379.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone Sp 6C6
All lanes : Anti-Spastin antibody [Sp 6C6] (ab77144) at 1/1500 dilutionLane 1 : Human induced pluripotent stem cell derived neuron whole cell lysateLane 2 : Human induced pluripotent stem cell derived neuron whole cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated goat anti-mouse IgG polyclonal at 1/10 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Spastin Antibody (clone 2F5) IHC-plus™ - LS-B6150 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2F5
Anti-SPAST / Spastin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6150 concentration 5 ug/ml.

Anti-Spastin Antibody (aa130-142) - LS-C132151 from LifeSpan Bioscience

Prices $495.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal

Anti-Spastin Antibody (aa561-573) - LS-C132152 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Spastin Antibody (aa130-142) - LS-C42156 from LifeSpan Bioscience

Prices $395.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
Human brain lysate probed with Rabbit anti-Human SPASTIN (LS-C42156).

Anti-Spastin Antibody (aa361-410) - LS-C201705 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human MCF7. SPAST antibody dilution 1.0 ug/ml.

Spastin Antibody (2F5) - H00006683-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2F5
Western Blot: Spastin Antibody (2F5) [H00006683-M02] - SPAST monoclonal antibody (M02), clone 2F5. Analysis of SPAST expression in Jurkat.

Spastin Antibody - NBP1-90230 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Spastin Antibody [NBP1-90230] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Spastin Antibody - NBP1-46433 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Spastin Antibody - NB100-62922 from Novus Biologicals

Prices $299.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Spastin Antibody [NB100-62922] - Human brain lysate probed with RABBIT ANTI HUMAN SPASTIN

Spastin Antibody (3A12) - H00006683-M01 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3A12
ELISA: Spastin Antibody (3A12) [H00006683-M01] - Detection limit for recombinant GST tagged SPAST is approximately 0.1ng/ml as a capture antibody.

Spastin Antibody - H00006683-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

FSP/Spastin Polyclonal Antibody - bs-1770R from Bioss

Prices $279.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded rat brain labeled with Anti-FSP/Spastin Polyclonal Antibody, Unconjugated (bs-1770R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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