All lanes : Anti-Spata18 antibody (ab107702) at 1 µg/mlLane 1 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 2 : Testis (Rat) Tissue LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Spata18 antibody (ab107702) at 1 µg/ml (Milk blocking - 3%)Lane 1 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 2 : Testis (Rat) Tissue LysateLane 3 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257) with Immunizing peptide at 1 µg/mlLane 4 : Testis (Rat) Tissue Lysate with Immunizing peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of Spata18 staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107702, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.