SPATA18

Gene Symbol SPATA18
Entrez Gene 132671
Alt Symbol Mieap, SPETEX1
Species Human
Gene Type protein-coding
Description spermatogenesis associated 18
Other Description mitochondria-eating protein|spermatogenesis associated 18 homolog|spermatogenesis-associated protein 18
Swissprots Q8N7D7 B4E2R0 Q8IY48 Q8TC71 E5RLK1
Accessions EAX05432 EAX05433 Q8TC71 AB465501 BAJ53738 AB465502 BAJ53739 AI339078 AK057468 BAB71501 AK098625 BAC05356 AK304386 BAG65222 AK314799 BC025396 AAH25396 BC037886 AAH37886 BU683915 BU930386 CK904112 DC404003 XM_005265716 XP_005265773 XM_005265717 XP_005265774 XM_005265718 XP_005265775 XM_011534369 XP_011532671 XM_011534370 XP_011532672 XM_011534371 XP_011532673 XM_011534372 XP_011532674 XR_941044 NM_001297608 NP_001284537 NM_145263 NP_660306 NR_123728
Function Key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage. Mediator of mitochondrial protein catabolic process (also named MALM) by mediating the degradation of damaged proteins inside mitochondria by promoting the accumulation in the mitochondrial matrix of hydrolases that are characteristic of the lysosomal lumen. Also involved in mitochondrion degradation of damaged mitochondria by promoting the formation of vacuole-like structures (named MIV), which engulf and degrade unhealthy mitochondria by accumulating lysosomes. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. {ECO:0000269|PubMed:21264221, ECO:0000269|PubMed:21264228, ECO:0000269|PubMed:22292033}.
Subcellular Location Cytoplasm. Mitochondrion outer membrane. Note=Localizes to the cytoplasm under normal conditions. Relocalizes to mitochondrion outer membrane following cellular stress. Colocalizes with BNIP3 and BNIP3L at the mitochondrion outer membrane.

SPATA18 (C-16) - sc-165544 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SPATA18 (K-13) - sc-165545 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SPATA18 (N-13) - sc-165547 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Spata18 antibody - ab107702 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Spata18 antibody (ab107702) at 1 µg/mlLane 1 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 2 : Testis (Rat) Tissue LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Spata18 antibody - ab116658 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Spata18 antibody (ab116658) at 1/100 dilution + NCI-H292 cell lysate at 15 µg

Anti-Spata18 antibody [EPR13704] - ab180154 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13704
All lanes : Anti-Spata18 antibody [EPR13704] (ab180154) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : LnCaP cell lysateLane 4 : HT1080 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Spata18 antibody - N-terminal - ab190116 from Abcam

Prices $407.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal
All lanes : Anti-Spata18 antibody - N-terminal (ab190116) at 1 µg/mlLane 1 : Rat lung tissue lysateLane 2 : Rat lung tissue lysate with blocking peptide

Anti-SPATA18 / MIEAP Antibody (aa92-120, APC) - LS-C241155 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPATA18 / MIEAP Antibody (aa92-120, AP) - LS-C241156 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPATA18 / MIEAP Antibody (aa92-120, Biotin) - LS-C241157 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPATA18 / MIEAP Antibody (aa92-120, FITC) - LS-C241158 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPATA18 / MIEAP Antibody (aa92-120, PE) - LS-C241162 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPATA18 / MIEAP Antibody (aa92-120, Azide-free, HRP) - LS-C248232 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPATA18 / MIEAP Antibody (aa91-120) - LS-C325310 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPATA18 / MIEAP Antibody (N-Terminus) - LS-C345105 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of SPATA18 Antibody with human Stomach Tumor lysate.

Anti-SPATA18 / MIEAP Antibody (N-Terminus) IHC-plus™ - LS-B9604 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal
Anti-SPATA18 / MIEAP antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9604 concentration 5 ug/ml.

Anti-SPATA18 / MIEAP Antibody (aa92-120) - LS-C161427 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
SPATA18 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

SPATA18 Antibody - NBP1-81984 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: SPATA18 Antibody [NBP1-81984] - Staining of human fallopian tube shows rootlet positivity in glandular cells.

Anti-SPATA18 antibody produced in chicken - SAB3500696 from Sigma-Aldrich

Prices $341.00
Sizes 100 µg
Host Chicken
<B>Immunoblotting</B><BR/>Anti-SPATA18; <B>Cat. No. SAB3500696</B>; Western blot analysis of SPATA18 in Rat lung tissue lysate with SPATA18 antibody at 1 μ/mL in (A) the absence and (B) the presence of blocking peptide.

Anti-SPATA18 (191-205) antibody produced in rabbit - SAB1104009 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human SPATA18 was separated on SDS-PAGE, blotted with Anti-SPATA18 (191-205) (<B>Cat. No. SAB1104009</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000 <BR/>2. Antibody dilution 1:1,000 + SPATA18 immunizing peptide (human, 191-205)

ANTI-SPATA18 (N-TERM) antibody produced in rabbit - SAB1304208 from Sigma-Aldrich

Prices $326.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>SPATA18 (N-term): <B>Cat. No. SAB1304208</B>: SPATA18 in Human testis tissue with SPATA18 antibody (N-term) at 10 μg/mL.

Anti-SPATA18 antibody produced in rabbit - HPA036854 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-SPATA18: <B>Cat. No. HPA036854</B>: Immunohistochemical staining of human nasopharynx shows strong cytoplasmic and membranous positivity in respiratory epithelial cells.

SPATA18 Antibody - middle region (ARP52838_P050) - ARP52838_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SPATA18-Antibody-N-term - AP13960a from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
SPATA18 Antibody (N-term) (Cat. #AP13960a) western blot analysis in NCI-H292 cell line lysates (15ug/lane).This demonstrates the SPATA18 antibody detected SPATA18 protein (arrow).

SPATA18 Antibody - N-terminal region (OAAB10258) - OAAB10258 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
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