All lanes : Anti-Spata18 antibody [EPR13704] (ab180154) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : LnCaP cell lysateLane 4 : HT1080 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Spata18 with ab180154 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counter stained with Dapi.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Spata18 with ab180154 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.