All lanes : Anti-Sphingomyelin Synthase 2 antibody - C-terminal (ab189443) at 1/1000 dilutionLane 1 : MCF7 cell lysateLane 2 : CEM cell lysateLane 3 : NCI-H460 cell lysateLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue, labeling Sphingomyelin Synthase 2 with ab189443 at 5 µg/ml, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adrenal tissue, labeling Sphingomyelin Synthase 2 with ab189443 at 5 µg/ml.
Flow cytometric analysis of permeabilized MCF7 cells labeling Sphingomyelin Synthase 2 with ab189443 at 1/10 dilution (right histogram), compared with a negative control cell (left histrogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.