Anti-sphingosine-1-phosphate phosphatase 1 antibody
| Name | Anti-sphingosine-1-phosphate phosphatase 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab129253 |
| Prices | $370.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 12-42 of the Human Sphingosine-1-phosphate phosphatase 1 protein (Q9BX95) |
| Description | Rabbit Polyclonal |
| Gene | SGPP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab129253 stained JEG3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab129253 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-sphingosine-1-phosphate phosphatase 1 antibody (ab129253) at 1/100 dilution + NCI-H292 cell line lysates at 35 µg
![ab129253, at 1/10 to 1/50, staining sphingosine-1-phosphate phosphatase 1 in Human placenta tissue followed by DAB staining by immunohistochemistry [Formalin fixed and Paraffin Embedded Tissues (IHC-P)].](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/4335_sphingosine-1-phosphate-phosphatase-1-Primary-antibodies-ab129253-3.jpg)
ab129253, at 1/10 to 1/50, staining sphingosine-1-phosphate phosphatase 1 in Human placenta tissue followed by DAB staining by immunohistochemistry [Formalin fixed and Paraffin Embedded Tissues (IHC-P)].
Product References
Loss of the neuroprotective factor Sphingosine 1-phosphate early in Alzheimer's - Loss of the neuroprotective factor Sphingosine 1-phosphate early in Alzheimer's
Couttas TA, Kain N, Daniels B, Lim XY, Shepherd C, Kril J, Pickford R, Li H, Garner B, Don AS. Acta Neuropathol Commun. 2014 Jan 23;2:9.
miRNA-95 mediates radioresistance in tumors by targeting the sphingolipid - miRNA-95 mediates radioresistance in tumors by targeting the sphingolipid
Huang X, Taeb S, Jahangiri S, Emmenegger U, Tran E, Bruce J, Mesci A, Korpela E, Vesprini D, Wong CS, Bristow RG, Liu FF, Liu SK. Cancer Res. 2013 Dec 1;73(23):6972-86.