All lanes : Anti-SPIB antibody [4G5] (ab135238) at 1/500 dilutionLane 1 : A549 cell lysateLane 2 : PC3 cell lysateLane 3 : NIH 3T3 cell lysate
Immunofluorescent analaysis of HeLa cells labelling SPIB with ab135238 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of NIH 3T3 cells labelling SPIB with ab135238 at 1/200 dilution (green). Negative control (red).
Immunohistochemical analysis of Paraffin-embedded Human ovarian cancer tissue labelling SPIB with ab135238 at 1/200 dilution followed by DAB staining.
Immunohistochemical analysis of Paraffin-embedded Human esophageal cancer tissue labelling SPIB with ab135238 at 1/200 dilution followed by DAB staining.