Anti-SPRED1 antibody
| Name | Anti-SPRED1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab62911 |
| Prices | $378.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Human |
| Antigen | Synthetic peptide from the C-terminal region of human SPRED1, conjugated to KLH |
| Description | Rabbit Polyclonal |
| Gene | SPRED1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab62911 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab62911 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-SPRED1 antibody (ab62911) at 1/100 dilutionLane 1 : 293 cell lysates nontransfectedLane 2 : 293 cell lysates transiently transfected with the SPRED1 geneLysates/proteins at 2 µg per lane.