SPRED1

Gene Symbol SPRED1
Entrez Gene 161742
Alt Symbol NFLS, PPP1R147, hSpred1, spred-1
Species Human
Gene Type protein-coding
Description sprouty-related, EVH1 domain containing 1
Other Description protein phosphatase 1, regulatory subunit 147|sprouty-related, EVH1 domain-containing protein 1|suppressor of Ras/MAPK activation
Swissprots Q8N256 Q05D53 B2RPJ8 Q7Z699
Accessions CCQ43858 EAW92368 Q7Z699 AK091222 BAC03614 AK095219 AY299089 AAP59414 BC018015 AAH18015 BC137480 AAI37481 BC137481 AAI37482 BX538234 DA405650 XM_005254202 XP_005254259 XM_005254203 XP_005254260 XM_011521288 XP_011519590 XM_011521289 XP_011519591 XM_011521290 XP_011519592 NM_152594 NP_689807
Function Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow (By similarity). {ECO:0000250}.
Subcellular Location Cell membrane {ECO:0000269|PubMed:16115197}; Peripheral membrane protein {ECO:0000269|PubMed:16115197}. Membrane, caveola {ECO:0000269|PubMed:16115197}; Peripheral membrane protein {ECO:0000269|PubMed:16115197}. Nucleus {ECO:0000269|PubMed:16115197}. Note=Localized in cholesterol-rich membrane raft/caveola fractions.
Tissue Specificity Weakly expressed in embryonic cell line HEK293. {ECO:0000269|PubMed:15580519}.
Top Pathways Jak-STAT signaling pathway

SPRED1 (M23-P2G3) - sc-101392 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SPRED1 (M23-P2G3): sc-101392. Western blot analysis of SPRED1 expression in HeLa whole cell lysate.

SPRED1 (E-5) - sc-393198 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SPRED1 (E-5): sc-393198. Western blot analysis of SPRED1 expression in HeLa whole cell lysate (A) and human hippocampus tissue extract (B).

SPRED1 (H-112) - sc-98290 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SPRED1 (H-112): sc-98290. Immunoperoxidase staining of formalin fixed, paraffin-embedded human upper stomach tissue showing cytoplasmic and nuclear staining of glandular cells.

SPRED1 (S-18) - sc-324354 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SPRED1 (T-17) - sc-324353 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SPRED1 (Y-24) - sc-130881 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
SPRED1 (Y-24): sc-130881. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing cytoplasmic localization.

Anti-SPRED1 antibody - ab77079 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-SPRED1 antibody (ab77079) at 1 µg/mlLane 2 : Anti-SPRED1 antibody (ab77079) at 2 µg/mlLane 1 : Human brain tissue lysateLane 2 : Human brain tissue lysateLysates/proteins at 15 µg per lane.

Anti-SPRED1 antibody - ab191278 from Abcam

Prices $384.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling SPRED1 with ab191278 at 1/75 dilution.

Anti-SPRED1 antibody - ab62911 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab62911 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab62911 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SPRED1 antibody [M23-P2G3] - ab64740 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone M23-P2G3
All lanes : Anti-SPRED1 antibody [M23-P2G3] (ab64740) at 10 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 4 : Brain (Rat) Tissue Lysate Lane 5 : Brain (Mouse) Tissue Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-SPRED1 Antibody (clone 462CT2.5.1, AP) - LS-C232247 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 462CT2.5.1

Anti-SPRED1 Antibody (clone 462CT2.5.1, APC) - LS-C232249 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 462CT2.5.1

Anti-SPRED1 Antibody (clone 462CT2.5.1, Biotin) - LS-C232250 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 462CT2.5.1

Anti-SPRED1 Antibody (clone 462CT2.5.1, FITC) - LS-C232251 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 462CT2.5.1

Anti-SPRED1 Antibody (clone 462CT2.5.1, PE) - LS-C232254 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 462CT2.5.1

Anti-SPRED1 Antibody (AP) - LS-C241210 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPRED1 Antibody (APC) - LS-C241211 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPRED1 Antibody (Biotin) - LS-C241212 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPRED1 Antibody (FITC) - LS-C241213 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPRED1 Antibody (PE) - LS-C241214 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPRED1 Antibody (aa309-339, APC) - LS-C241215 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPRED1 Antibody (Internal) - LS-C83935 from LifeSpan Bioscience

Prices $345.00, $1,035.00
Sizes 100 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Western blot of Spred1 in mouse brain tissue lysate with Spred1 antibody at (A) 1 and (B) 2 ug/ml.

Anti-SPRED1 Antibody (aa309-339, AP) - LS-C241216 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPRED1 Antibody - LS-C157081 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
SPRED1 Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The SPRED1 antibody detected the SPRED1 protein (arrow).

Anti-SPRED1 Antibody (aa309-339, Biotin) - LS-C241217 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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