Immunofluorescence analysis of paraformaldehyde-fixed SH-SY5Y cells labeling Sprouty 2 with ab180527 at 1/1000 dilution. Goat anti-rabbit IgG (Dylight 488) at 1/250 dilution was used as the secondary antibody. The slide is counterstained with Dapi.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Sprouty 2 with ab180527 at 1/50 dilution. The slide is counterstained with Hematoxylin.
All lanes : Anti-Sprouty 2 antibody [EPR4318(2)(B)] (ab180527) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : SH-SY5Y cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Sprouty 2 antibody [EPR4318(2)(B)] (ab180527) at 1/2000 dilutionLane 1 : HT-29 cell lysateLane 2 : PC-3 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution