Western blot analysis on immunoprecipitation pellet from MCF-7 cells lysate (lane 1) or 1XPBS (negative control, lane 2) using ab176337 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow cytometric analysis of permeabilized K562 cells labeling Sprouty 4 with ab176337 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunofluorescent analysis of HeLa cells labeling Sprouty 4 with ab176337 at 1/50 dilution (green). DAPI nuclear staining (blue).
All lanes : Anti-Sprouty 4 antibody [EPR12127] (ab176337) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : MCF7 cell lysateLane 3 : HeLa cell lysateLane 4 : K562 cell lysateLysates/proteins at 10 µg per lane.