Anti-Spt6 antibody
| Name | Anti-Spt6 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab32820 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IP WB |
| Species Reactivities | Human, Mouse, Zebrafish |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1150 - 1250 of Human Spt6 |
| Description | Rabbit Polyclonal |
| Gene | SUPT6H |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : MarkerLanes 2 - 4 : Anti-Spt6 antibody (ab32820) at 1/500 dilutionLane 5 : High Mark markerLane 1 : As aboveLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 20 µgLane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µgLane 5 : As aboveSecondaryLanes 2 - 4 : IR Dye 680 Conjugated Goat anti Rabbit IgG (H+L) at 1/21250 dilutionPerformed under reducing conditions.
Image courtesy of Human Protein Atlas ab32820 staining Spt6 in human gall bladder, showing a distinct and strong staining pattern in glandular cells. Paraffin embedded human skin tissue was incubated with ab32820 (1/30 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab32820 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.
![Spt6 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Spt6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32820.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 230kDa: Spt6](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/4670_Spt6-Primary-antibodies-ab32820-2.jpg)
Spt6 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Spt6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32820.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 230kDa: Spt6
Product References
Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR. - Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR.
Nakamura M, Basavarajaiah P, Rousset E, Beraud C, Latreille D, Henaoui IS, Lassot I, Mari B, Kiernan R. Retrovirology. 2012 Feb 8;9:13.
Chromatin reassembly factors are involved in transcriptional interference - Chromatin reassembly factors are involved in transcriptional interference
Gallastegui E, Millan-Zambrano G, Terme JM, Chavez S, Jordan A. J Virol. 2011 Apr;85(7):3187-202.
Yeast genetic analysis reveals the involvement of chromatin reassembly factors in - Yeast genetic analysis reveals the involvement of chromatin reassembly factors in
Vanti M, Gallastegui E, Respaldiza I, Rodriguez-Gil A, Gomez-Herreros F, Jimeno-Gonzalez S, Jordan A, Chavez S. PLoS Genet. 2009 Jan;5(1):e1000339.
Identification of novel SHPS-1-associated proteins and their roles in regulation - Identification of novel SHPS-1-associated proteins and their roles in regulation
Shen X, Xi G, Radhakrishnan Y, Clemmons DR. Mol Cell Proteomics. 2009 Jul;8(7):1539-51.