Anti-SQSTM1 / p62 antibody
| Name | Anti-SQSTM1 / p62 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab91526 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IHC-P ICC/IF ICC/IF IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A 14 amino acid peptide from near the Carboxy terminus of Human SQSTM1/ p62 (GenBank accession no |
| Description | Rabbit Polyclonal |
| Gene | SQSTM1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-SQSTM1 / p62 antibody (ab91526) at 1 µg/mlLane 2 : Anti-SQSTM1 / p62 antibody (ab91526) at 2 µg/mlLane 1 : Human spleen tissue lysateLane 2 : Human spleen tissue lysateLysates/proteins at 15 µg per lane.
ICC/IF image of ab91526 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91526, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab91526 at a 1/500 dilution staining SQSTM1/ p62 in wild type murine embryonic fibroblasts by Immunocytochemistry/ Immunofluorescence.
Immunofluorescence of SQSTM1 in Rat Spleen cells using ab91526 at 20 ug/ml.
Immunohistochemical analysis of mouse VCPR155H/R155H quadricep tissue, staining SQSTM1 / p62 (green) with ab91526. Mice were transcardially perfused with 4% paraformaldehyde before muscle tissue was frozen and sectioned. Tissue was incubated with primary antibody before detection with a fluorescein-conjugated anti-rabbit secondary antibody. Nuclei were stained with DAPI (blue).
Product References
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